| For medical practitioners and the general public - Cholesterol Journal Article Catalog. |
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| Effects of dietary combination of chromium and biotin on egg production, serum metabolites, and egg yolk mineral and cholesterol concentrations in heat-distressed laying quails Sahin, K., M. Onderci, et al. (2004), Biol Trace Elem Res 101(2): 181-92. Abstract: Chromium picolinate is used in the poultry diet because of its antistress effects in addition to the fact that the requirement for it is increased during stress. This study was conducted to determine if the negative effects of high ambient temperature (34 degrees C) on egg production, egg quality, antioxidant status, and cholesterol and mineral content of egg yolk could be alleviated by combination of chromium picolinate and biotin (0.6/2.0; Diachrome, as formulated by Nutrition 21 Inc.), in laying Japanese quails (Coturnix coturnix japanica). Quails (n= 240; 50 d old) were divided into 8 groups, 30 birds per group. The quails were fed either a basal diet or the basal diet supplemented with 2, 4, or 8 mg of Diachrome/kg diet. Birds were kept at 22 degrees C and 53% relative humidity (RH). At 14 wk of age, the thermoneutral (TN) group remained in the same temperature as at the beginning of experiment, whereas the heat stress (HS) group was kept in an environment-controlled room (34 degrees C and 41% RH) for 3 wk. Heat exposure decreased performance when the basal diet was fed (p = 0.001). Diachrome supplementation at 4 and 8 mg/kg diet, increased feed intake (p = 0.05), egg production (p = 0.05), feed efficiency (p = 0.01), egg weight (p = 0.05), and Haugh unit (p = 0.01) in quails reared under heat stress conditions. Heat exposure increased concentrations of serum malondialdehyde (MDA) (p = 0.001), glucose, and cholesterol (p = 0.01), which were elevated by supplemental Diachrome (p < or = 0.05). Egg yolk Cr, Zn, and Fe (p = 0.01) concentrations increased linearly, whereas MDA and cholesterol concentrations decreased (p = 0.05) as dietary Diachrome supplementation increased in HS groups. Similar effects of supplementation on serum levels of glucose and cholesterol (p = 0.05) and egg yolk concentrations of cholesterol (p = 0.05) and Cr (p = 0.01) were observed in TN groups. No significant differences in other values were observed in the TN groups. Results of the present study suggest that supplementation with Diachrome protects the quail by reducing the negative effects of heat stress. |
| Effects of dietary conjugated linoleic acid on fatty acid composition and cholesterol content of hen egg yolks Szymczyk, B. and P. M. Pisulewski (2003), Br J Nutr 90(1): 93-9. Abstract: The main objectives of the present study were to determine the effect of dietary conjugated linoleic acid (CLA) isomers on the fatty acid composition and cholesterol content of egg-yolk lipids. Forty-five 25-week-old laying hens were randomly distributed into five groups of nine hens each and maintained in individual laying cages, throughout 12 weeks of the experiment. They were assigned to the five treatments that consisted of commercial layer diets containing 0, 5, 10, 15 or 20 g pure CLA/kg. Feed intake of hens varied little and insignificantly. Egg mass was uniformly lower (P<0.05) in the hens fed the CLA-enriched diets. Feed conversion efficiency, when expressed per kg eggs, was impaired (P<0.05), although without obvious relation to the dietary CLA concentration. Feeding the CLA-enriched diets resulted in gradually increasing deposition of CLA isomers (P<0.01) in egg-yolk lipids. Saturated fatty acids were increased (P<0.01) and monounsaturated fatty acids decreased (P<0.01). Polyunsaturated fatty acids (PUFA), when expressed as non-CLA PUFA, were also significantly decreased (P<0.01). The most striking effects (P<0.01) were observed for palmitic (16: 0) and stearic (18: 0) acids, which increased from 23.6 to 34 % and from 7.8 to 18 %, respectively. On the other hand, oleic acid (18: 1n-9) decreased from 45.8 to 24.3 %. Among non-CLA PUFA, linoleic (18: 2n-6) and alpha-linolenic (18: 3n-3) acids were strongly (P<0.01) decreased, from 14.2 to 7.7 % and from 1.3 to 0.3 %, respectively. The same was true for arachidonic (20:4n-6) and docosahexaenoic (22: 6n-3) acids. The cholesterol content of egg yolks, when expressed in mg/g yolk, was not affected by the dietary CLA concentrations. In conclusion, unless the adverse effects of CLA feeding to laying hens on the fatty acid profile of egg yolks are eliminated, the CLA-enriched eggs cannot be considered functional food products. |
| Effects of dietary copper on production and egg cholesterol content in laying hens Balevi, T. and B. Coskun (2004), Br Poult Sci 45(4): 530-4. Abstract: 1. This experiment was carried out to determine the effects of diets supplemented with different amounts of copper on egg production, food intake, food conversion ratio, egg weight, damaged egg ratio, specific gravity, mortality and cholesterol concentration in yolk. The experiment lasted 90 d and 400 Hisex-Brown hens, aged 27 weeks at the start of the study, were used. 2. There were no statistically significant effects of supplementary copper on egg production, food intake or food conversion efficiency. 3. There were no statistically significant differences between the groups in terms of damaged eggs, egg weight, specific gravity and live weight. 4. At the end of the experiment, the lowest yolk cholesterol concentrations were obtained in the 150 kg/kg copper group and the greatest concentrations were in the control group. 5. Consequently, the use of supplementary copper to provide 150 kg/kg in poultry diets was concluded to decrease yolk cholesterol concentrations without any effect on production performance. |
| Effects of dietary copper source and concentration on carcass characteristics and lipid and cholesterol metabolism in growing and finishing steers Engle, T. E., J. W. Spears, et al. (2000), J Anim Sci 78(4): 1053-9. Abstract: We conducted an experiment to determine the effects of dietary copper (Cu) source and level on carcass characteristics, longissimus muscle fatty acid composition, and serum and muscle cholesterol concentrations in steers. Sixty Angus and Angus x Hereford steers were stratified by weight and initial liver Cu concentration within a breed and randomly assigned to treatments. Treatments consisted of: 1) control (no supplemental Cu); 2) 20 mg Cu/kg DM from Cu sulfate (CuSO4); 3) 40 mg Cu/kg DM from CuSO4; 4) 20 mg Cu/kg DM from Cu citrate; 5) 20 mg Cu/kg DM from Cu proteinate; and 6) 20 mg Cu/kg DM from tribasic Cu chloride. A corn silage-soybean meal-based diet was fed for 56 d. Steers were then switched to a high-concentrate diet. Equal numbers (n = 5) of steers per treatment were slaughtered after receiving the finishing diets for either 101 or 121 d. Serum cholesterol was not affected by treatment during the growing phase but was decreased (P <.05) in steers supplemented with Cu by d 84 of the finishing period and remained lower (P <. 05) at subsequent sampling periods. Longissimus muscle cholesterol concentration tended to be reduced (P <.11) by Cu supplementation. Hot carcass weight and backfat were lower (P <.05) in animals receiving supplemental Cu. However, Cu-supplemented and control steers had similar marbling scores. Longissimus muscle polyunsaturated fatty acid concentrations (18:2 and 18:3) were increased (P <.07) and saturated fatty acid concentrations tended (P <. 11) to be reduced by Cu supplementation. These results indicate that as little as 20 mg of supplemental Cu/kg diet can reduce backfat and serum cholesterol and increase muscle polyunsaturated fatty acids in steers fed high-concentrate diets. |
| Effects of dietary corn and olive oil versus coconut fat on biliary cholesterol secretion in rats Smit, M. J., H. Wolters, et al. (1994), Int J Vitam Nutr Res 64(1): 75-80. Abstract: We have studied the effects of dietary corn and olive oil versus coconut fat on bile formation and fluidity of hepatic plasma membranes in rats. After 4 weeks of feeding the purified diets containing 9% (w/w) of the test fats, there was no difference in plasma cholesterol concentration between the dietary groups. The amount of free and esterified cholesterol in the liver was significantly higher in rats fed either corn oil or olive oil as compared with coconut fat. In the rats fed olive oil, but not in those fed corn oil this was associated with lower rates of biliary phospholipid excretion. Bile flow was not differently influenced by the three dietary fats. Hepatic plasma membranes of the rats fed corn or olive oil contained more cholesterol and less phospholipids than those on coconut fat, which was, however, not accompanied by changes in fluidity of the membranes. These results indicate that in rats the type of dietary fat can induce considerable changes in hepatic cholesterol metabolism without affecting plasma cholesterol concentrations, and without consistent effects on biliary cholesterol secretion. |
| Effects of dietary crude palm oil, fish oil and their association on cholesterol and lipoprotein constants in rats which could be beneficial in humans Niyongabo, A., A. Youyou, et al. (1999), Int J Vitam Nutr Res 69(5): 330-6. Abstract: The aim was first to examine the differential effects of crude and refined palm oil (CPO and RPO) on the lipid and lipoprotein constants of plasma in rats and to compare the effect of crude palm oil to that of fish oil. Secondarily, it was to know whether one can take advantage from the association of CPO with FO. Twenty-four-day-old weaning rats were divided into five experimental groups, each receiving a purified diet containing 10% oil as either a single oil or an equal amount of two oils. After a feeding period of 36 days, the main results were as follows. As compared to the rats fed the RPO diet, those fed the CPO diet had lower total cholesterol, LDL-C, VLDL-C and apoB and higher HDL-C/LDL-C and apoA1/apoB ratios. Those fed the FO diet had only lower VLDL-C and triglycerides and higher HDL-C and HDL-C/LDL-C ratio. Whereas FO associated with RPO in the same diet had the same effect as FO alone, FO associated with CPO tends to reinforce the effect of CPO. This is particularly true for the effects on apoB and apoA1 which were found to be synergistically depressed and enhanced, respectively. Given the role played by these biological constants as predictors of CVD in humans, and in spite of the fact that these predictors are not relevant in rats, these results would suggest the potential interest of CPO or the association of CPO with FO in human nutrition. |
| Effects of dietary defatted squid on cholesterol metabolism and hepatic lipogenesis in rats Tanaka, K., I. Ikeda, et al. (2001), Lipids 36(5): 461-6. Abstract: Male Sprague-Dawley rats were fed a cholesterol-free (Exp. 1) or cholesterol-supplemented (Exp. 2) diet containing 20% casein (control group) or 15% defatted squid and 5% casein (defatted squid group), as protein, for 14 d. Serum and hepatic cholesterol concentrations were lower in rats fed defatted squid than in those fed casein in both cholesterol-free (-20%, P < 0.05 and -15%, P < 0.05, respectively) and cholesterol-supplemented (-25%, P < 0.05 and -15%, P < 0.05, respectively) diets. Hepatic triglyceride concentration was lower in the defatted squid than in the control groups in both cholesterol-free (-51%, P< 0.05) and cholesterol-supplemented diets (-38%, P < 0.01). The activities of cytosolic fatty acid synthase and the NADPH-generating enzymes, malic enzyme and glucose-6-phosphate dehydrogenase, in the liver were lower in the defatted squid than in the control groups in both cholesterol-free (-21%, P< 0.01, -33%, P < 0.05, and -33%, P < 0.01, respectively) and cholesterol-supplemented diets (-34%, P < 0.05, -57%, P < 0.05, and -67%, P < 0.05, respectively). The activity of mitochondrial carnitine palmitoyltransferase in the liver was comparable between the control and defatted squid groups. The activity of Mg2+-dependent phosphatidate phosphohydrolase in the liver cytosol was lower in the defatted squid (-9%, P < 0.05) than in the control groups only in the cholesterol-free diet. Fecal excretion of total steroids was stimulated by the feeding of defatted squid in both cholesterol-free (+77%, P < 0.005) and cholesterol-supplemented diets (+29%, P < 0.01). These results suggest that the nonlipid fraction of squid exerts a hypocholesterolemic effect by increasing the excretion of total steroids in feces. The fraction also induces a triglyceride-lowering activity in the liver by decreasing hepatic lipogenesis. |
| Effects of dietary excess amino acids on the concentrations of cholesterol, alpha-tocopherol, ascorbic acid, and copper in serum and tissues of rats Katayama, T., J. Hayashi, et al. (1990), J Nutr Sci Vitaminol (Tokyo) 36(5): 485-95. Abstract: An experiment was conducted with growing rats to investigate the effects of feeding excessive specific L-amino acids for 8 days on serum and tissue cholesterol, alpha-tocopherol, ascorbic acid, and copper, and on liver microsomal cytochrome P-450. To a 10% casein diet were added 4% L-methionine, 5% L-cystine, 5% L-histidine, 5% L-threonine, 5% L-tryptophan, 5% L-phenylalanine, 5% L-tyrosine, 6% L-valine, 7% L-isoleucine, 7% L-lysine, or 8% L-leucine. Excessive cystine and histidine increased serum cholesterol and alpha-tocopherol. Excessive cystine and methionine increased liver and kidney alpha-tocopherol and ascorbic acid. Excessive tyrosine and phenylalanine caused a marked increase in serum copper and ceruloplasmin activity, whereas excessive cystine, methionine, and histidine caused a decrease in the ceruloplasmin activity. Excessive histidine increased liver cytochrome P-450, whereas excessive tyrosine markedly decreased liver cytochrome P-450. |
| Effects of dietary fat and fatty acids on coronary artery disease risk and total and lipoprotein cholesterol concentrations: epidemiologic studies Caggiula, A. W. and V. A. Mustad (1997), Am J Clin Nutr 65(5 Suppl): 1597S-1610S. Abstract: Because of their large sample sizes, epidemiologic studies can provide important data on the relation between diet, specifically dietary fat and fatty acids, and lipid and lipoprotein concentrations as well as the incidence of coronary artery disease. Although correlation coefficients vary widely (from 0.84 to 0.01) and are frequently low between dietary variables and coronary artery disease or lipids and lipoprotein fractions in the studies reviewed here, intakes of saturated fatty acids and dietary cholesterol are generally positively correlated with blood cholesterol in men and women. Associations between other fatty acids are less consistent and may be related to the considerable differences in these studies in dietary methodologies used, databases used for analyses, and homogeneity of intakes within populations. Relatively few data are available for women, cultural minorities within the United States, or young or elderly populations. However, the trends observed in the United States, ie, lower rates of coronary artery disease as well as lower reported intakes of both total and saturated fats, support the relations observed in the epidemiologic studies. |
| Effects of dietary fat and oxidized cholesterol on gene expression in rat liver as assessed by cDNA expression array analysis Ringseis, R. and K. Eder (2005), Eur J Nutr 44(4): 231-41. Abstract: BACKGROUND: Specific oxysterols acting as ligands for nuclear transcription factors were shown to affect expression of genes involved in lipid metabolism. However, the various biological effects of oxysterols such as cytotoxicity, atherogenicity or mutagenicity suggest that other genes may be also affected by oxysterols than lipid metabolism. AIM OF THE STUDY: The present study was conducted to investigate the effects of dietary oxidized cholesterol containing significant amounts of oxysterols and its interactions with different dietary fats on gene expression profiles as assessed by DNA array technology in rats. METHODS: 54 male Sprague-Dawley rats were assigned to six groups and were fed six semisynthetic diets, which varied in the type of dietary fat (coconut oil vs. salmon oil) and dietary cholesterol (none cholesterol vs. 5 g unoxidized cholesterol/kg vs. 5 g oxidized cholesterol/kg). RESULTS: Changes in gene expression as observed in response to dietary oxidized cholesterol were strongly dependent on the type of fat. In the rats fed coconut oil, the expression of 7 genes (5 up- and 2 down-regulated) was altered by dietary oxidized cholesterol, while in the rats fed salmon oil, the expression of 50 genes (16 up- and 34 down-regulated) was altered. 29 genes (22 up- and 7 down-regulated) were identified as possible targets for an altered gene expression by dietary salmon oil as compared to dietary coconut oil. CONCLUSION: The present study showed that dietary oxidized cholesterol transcriptionally affects many genes involved in xenobiotic metabolism and stress response--an effect that was amplified by the administration of fish oil as dietary fat. |
| Effects of dietary fat and season on steroid hormonal profiles before parturition and on hormonal, cholesterol, triglycerides, follicular patterns, and postpartum reproduction in Brahman cows Lammoglia, M. A., S. T. Willard, et al. (1996), J Anim Sci 74(9): 2253-62. Abstract: Spring-calving Brahman cows (S) artificially inseminated to Brahman, Angus, or Tuli sires and fall-calving Brahman cows (F) naturally bred to Brahman were allotted randomly to receive 3.74% (LF; n = 9 S and 6 F), 5.20% (MF; n = 8 S and 6 F), or 6.55% dietary fat (HF; n = 8 S). Diets were formulated to contain differing fatty acid concentrations and to be isocaloric and isonitrogenous. Cows were bled and fed twice daily from 2 wk before expected calving date through d 21 after calving. Ultrasonography was performed on d 14 and 21 after calving. From d 21 to 90 after calving a sterile bull equipped with a chin-ball marker was placed with the cows to aid in estrus detection. In both seasons progesterone decreased (P <.01) and estradiol-17 beta increased (P <.01) as parturition approached. Cows receiving MF and HF had increased (P <.01) total numbers of follicles compared to LF cows, and cows receiving MF had larger (P <.01) follicles. During the spring, cows receiving HF and cows bred to Brahman or Tuli sires had longer (P <.01) gestation lengths. Progesterone concentrations before calving were affected (P <.01) by treatment x sire and estradiol-17 beta by a time x treatment interaction (P <.01). Cholesterol after calving was higher (P <.01) in HF cows than in LF or MF cows. In the fall, LF cows had heavier (P <.01) calves than cows receiving MF. Birth weight was also affected (P <.01) by treatment x sex of calf. Progesterone was affected (P <.01) by treatment x sex of calf. Estradiol-17 beta was affected (P <.01) by sex of calf and treatment x sex of calf. Across seasons, by d 90 after calving, 9 of 15 (60%) LF and 11 of 15 (73.3%) MF cows showed estrual behavior. Cows in the spring had increased (P <.01) numbers and larger follicles compared to the fall. In conclusion, dietary fat may influence steroid hormone concentrations before calving, calf birth weight and postpartum follicular populations; furthermore, follicular populations may also be influenced by season. |
| Effects of dietary fat on cholesterol movement between tissues in CBA/J and C57BR/cdJ mice Kuan, S. I., J. Stewart, et al. (1992), J Lipid Res 33(11): 1619-28. Abstract: Differences in dietary fats cause differences in cholesterol metabolism in mice. CBA/J mice are resistant to diet-induced hypercholesterolemia and atherosclerosis; they adjust hepatic hydroxymethyl-glutaryl-CoA reductase activity (HMGR) to maintain homeostasis; C57BR/cdJ mice are susceptible, but young animals are thought to maintain homeostasis by changing fecal excretion of sterols. Compartmental modelling of movement of 4-14Ccholesterol was used to analyze movement of cholesterol between serum and liver, heart, and carcass in mice fed 40 en% fat, polyunsaturated to saturated fatty acid ratio (P/S) = 0.24 (US74) or 30 en% fat, P/S = 1 (MOD). Dietary effects were quite pronounced, while strain effects were more subdued. The C57/cdJ animals appear to regulate the overall cholesterol balance by reducing synthesis, as do the CBA/J animals, even though synthesis is not reduced to the same degree as in the CBA/J animals. Both diet and strain influence the whole-animal turnover rate, with slower turnover occurring for C57BR/cdJ animals and animals fed the US74 diet. |
| Effects of dietary fats and cholesterol on liver lipid content and hepatic apolipoprotein A-I, B, and E and LDL receptor mRNA levels in cebus monkeys Hennessy, L. K., J. Osada, et al. (1992), J Lipid Res 33(3): 351-60. Abstract: The effects of the long-term administration of the dietary fats coconut oil and corn oil at 31% of calories with or without 0.1% (wt/wt) dietary cholesterol on plasma lipoproteins, apolipoproteins (apo), hepatic lipid content, and hepatic apoA-I, apoB, apoE, and low density lipoprotein (LDL) receptor mRNA abundance were examined in 27 cebus monkeys. Relative to the corn oil-fed animals, no significant differences were noted in any of the parameters of the corn oil plus cholesterol-fed group. In animals fed coconut oil without cholesterol, significantly higher (P less than 0.05) plasma total cholesterol (145%), very low density lipoprotein (VLDL) + LDL (201%) and high density lipoprotein (HDL) (123%) cholesterol, apoA-I (103%), apoB (61%), and liver cholesteryl ester (263%) and triglyceride (325%) levels were noted, with no significant differences in mRNA levels relative to the corn oil only group. In animals fed coconut oil plus cholesterol, all plasma parameters were significantly higher (P less than 0.05), as were hepatic triglyceride (563%) and liver apoA-I (123%) and apoB (87%) mRNA levels relative to the corn oil only group, while hepatic LDL receptor mRNA (-29%) levels were significantly lower (P less than 0.05). Correlation coefficient analyses performed on pooled data demonstrated that liver triglyceride content was positively associated (P less than 0.05) with liver apoA-I and apoB mRNA levels and negatively associated (P less than 0.01) with hepatic LDL receptor mRNA levels. Liver free and esterified cholesterol levels were positively correlated (P less than 0.05) with liver apoE mRNA levels and negatively correlated (P less than 0.025) with liver LDL receptor mRNA levels. Interestingly, while a significant correlation (P less than 0.01) was noted between hepatic apoA-I mRNA abundance and plasma apoA-I levels, no such relationship was observed between liver apoB mRNA and plasma apoB levels, suggesting that the hepatic mRNA of apoA-I, but not that of apoB, is a major determinant of the circulating levels of the respective apolipoprotein. Our data indicate that a diet high in saturated fat and cholesterol may increase the accumulation of triglyceride and cholesterol in the liver, each resulting in the suppression of hepatic LDL receptor mRNA levels. We hypothesize that such elevations in hepatic lipid content differentially alter hepatic apoprotein mRNA levels, with triglyceride increasing hepatic mRNA concentrations for apoA-I and B and cholesterol elevating hepatic apoE mRNA abundance. |
| Effects of dietary fatty acid composition on plasma cholesterol Khosla, P. and K. Sundram (1996), Prog Lipid Res 35(2): 93-132. Abstract: It should be clear from the preceding sections that the effects of dietary fatty acids on plasma lipids get more complicated the more we try to simplify them! We have presented one argument as to how different fatty acids may interact to impact human plasma lipids. This is by no means an endorsement that ours is the only argument. Nevertheless, a strong case can be made for 14:0 and 18:2 as being the key players in this scenario. The role of palmitic acid seems to be the most controversial. While clearly certain studies do indeed reveal 16:0 to be hypercholesterolemic relative to 18:1, the data from studies suggesting that it behaves similarly to 18:1 are equally compelling. What is certain is that it is erroneous to assume that 16:0 is the major cholesterol-raising SFA simply because it is the most abundant SFA in the diet. Clearly, 18:0 cannot be considered cholesterol-elevating. One is therefore left with the 12-16C SFA. However, 12:0 and 14:0 are only of concern if diets contain palm-kernel, coconut oil or dairy products as major dietary constituents. Accordingly one is left with 16:0 and its response is highly dependent on the metabolic status as well as the age of the subjects being used. While "elderly" hypercholesterolemic humans clearly benefit from decreased 16:0 (and all SFA) consumption, "younger" normocholesterolemic subjects fail to show such clear-cut effects. Additionally, the concomitant levels of dietary cholesterol and 18:2 also have a major bearing on the cholesterolemic response of 16:0 As far as guidelines for the general public are concerned, clearly for people with TC > 225 and LDL-C > 130 mg/dl and/or those who are overweight (i.e. those percieved to be at high risk), the primary emphasis should clearly be on reducing total fat consumption. Decreasing saturated fat consumption will invariably also lower dietary cholesterol consumption. The latter manouver will generally lower TC and LDL-C. Whether the reduction occurs because of the removal of 14:0, or 16:0 and/or dietary cholesterol is a mute point, since most dietary guidelines advocate curtailing intake of animal and dairy products, which will result in reductions of all the SFA. It remains to be established whether life-long adherence to the above dietary guidelines in those subjects with normal cholesterol levels and an absence of the other conventional risk factors for CHD, will result in a subsequent decrease in CHD risk. In the latest NCEP report 39 million Americans were targeted as those who would benefit from reductions in LDL-C, principally by dietary means. This is indeed a very high number. But that leaves almost 220 million Americans! For them the age old recommendation to consume a moderate fat load, maintain ideal body weight and eat a varied and balanced diet would still appear to be the most powerful advice. |
| Effects of dietary fatty acids and carbohydrates on the ratio of serum total to HDL cholesterol and on serum lipids and apolipoproteins: a meta-analysis of 60 controlled trials Mensink, R. P., P. L. Zock, et al. (2003), Am J Clin Nutr 77(5): 1146-55. Abstract: BACKGROUND: The effects of dietary fats on the risk of coronary artery disease (CAD) have traditionally been estimated from their effects on LDL cholesterol. Fats, however, also affect HDL cholesterol, and the ratio of total to HDL cholesterol is a more specific marker of CAD than is LDL cholesterol. OBJECTIVE: The objective was to evaluate the effects of individual fatty acids on the ratis of total to HDL cholesterol and on serum lipoproteins. DESIGN: We performed a meta-analysis of 60 selected trials and calculated the effects of the amount and type of fat on total:HDL cholesterol and on other lipids. RESULTS: The ratio did not change if carbohydrates replaced saturated fatty acids, but it decreased if cis unsaturated fatty acids replaced saturated fatty acids. The effect on total:HDL cholesterol of replacing trans fatty acids with a mix of carbohydrates and cis unsaturated fatty acids was almost twice as large as that of replacing saturated fatty acids. Lauric acid greatly increased total cholesterol, but much of its effect was on HDL cholesterol. Consequently, oils rich in lauric acid decreased the ratio of total to HDL cholesterol. Myristic and palmitic acids had little effect on the ratio, and stearic acid reduced the ratio slightly. Replacing fats with carbohydrates increased fasting triacylglycerol concentrations. CONCLUSIONS: The effects of dietary fats on total:HDL cholesterol may differ markedly from their effects on LDL. The effects of fats on these risk markers should not in themselves be considered to reflect changes in risk but should be confirmed by prospective observational studies or clinical trials. By that standard, risk is reduced most effectively when trans fatty acids and saturated fatty acids are replaced with cis unsaturated fatty acids. The effects of carbohydrates and of lauric acid-rich fats on CAD risk remain uncertain. |
| Effects of dietary fatty acids on signal transduction and membrane cholesterol content in rat platelets Heemskerk, J. W., M. A. Feijge, et al. (1995), Biochim Biophys Acta 1255(1): 87-97. Abstract: Previously, we have reported that dietary fatty acids can modify the thromboxane A2-dependent activation of rat platelets. Here, we present evidence that this dietary effect is part of a more general effect on platelet signal transduction, putatively involving structural changes in the platelet membranes. Four experiments were performed, where Wistar rats were fed with a high-fat diet enriched in either saturated, n-6 polyunsaturated or n-3 polyunsaturated fatty acids, or with a low-fat diet enriched in n-6 polyunsaturated fatty acids. The type of diet hardly influenced mean number of double bonds in the major platelet phospholipids. Platelet membranes from the rats fed with the saturated-fat diet had phospholipids with relatively high levels of arachidonate, but were low in cholesterol/phospholipid ratio. When compared to this diet group, platelets from other groups had an arachidonate content that was 21 to 47% lower and a cholesterol/phospholipid ratio 3 to 5% higher. The saturated-fat diet resulted in platelets that, in general, were less responsive to agonists than the platelets from other groups: with thrombin, collagen and thromboxane A2 analogue U46619, both early (shape change and phospholipase C-dependent rise in Ca2+i) and late (exocytosis and aggregation) responses were relatively low. However, platelet activation evoked by ADP was not influenced by diet type. When the cholesterol content of rat platelets was modified in vitro, it appeared that the early and late responses to thrombin and U46619 increased with the cholesterol/phospholipid ratio. Taken together, these results suggest that in rat platelets (i) the membrane cholesterol/phospholipid ratio can be modulated by a diet rich in saturated fatty acids, explaining, at least in part, the dietary effect on phospholipase C-mediated platelet activation, and (ii) relatively small changes in cholesterol content can have a more profound effect on platelet activation than substantial changes in arachidonate level. |
| Effects of dietary fish oil on biliary phospholipids and prostaglandin synthesis in the cholesterol-fed prairie dog Booker, M. L., T. E. Scott, et al. (1990), Lipids 25(1): 27-32. Abstract: Cholesterol gallstone formation in the prairie dog is accompanied by an increase in the percentage of biliary phospholipids containing arachidonic acid, and an increase in gallbladder prostaglandin (PG) synthesis, but the pathogenetic significance of these changes is unclear. Dietary supplementation with eicosapentaenoic acid (EPA), an omega-3 fatty acid which is commonly found in fish oil, decreases prostaglandin synthesis in some tissues by replacing arachidonic acid, and by competitively inhibiting prostaglandin synthesis. We studied the effect of dietary fish oil on gallbladder PG synthesis, and the relative abundance of various molecular species of phosphatidylcholines and phosphatidylethanolamines in bile and gallbladder epithelium in the cholesterol-fed prairie dog. Prairie dogs were maintained for 4 weeks on one of four diets: i) control, ii) cholesterol-supplemented (0.34%), iii) menhaden oil (50 g/kg chow), or iv) cholesterol plus menhaden oil. Supplementation with menhaden oil resulted in a replacement of arachidonic and linoleic acids with EPA and docosahexaenoic acids in the phospholipids of bile and gallbladder mucosa. In cholesterol-fed animals, supplementation with menhaden oil prevented increased gallbladder PG synthesis. Menhaden oil also reduced the incidence of cholesterol monohydrate crystals among cholesterol-fed animals (9/20 with cholesterol plus menhaden oil vs 21/22 with cholesterol alone), but the improvement could not clearly be attributed to decreased PG synthesis since supplementation with menhaden oil also increased the total phospholipid concentration in bile, and decreased the degree of cholesterol saturation. These results demonstrate that dietary supplementation with omega-3 fatty acids significantly influences biliary phospholipids, and decreases the incidence of cholesterol monohydrate crystal formation in this animal model. |
| Effects of dietary fish protein on plasma cholesterol and lipoproteins in animal modes and in humans Jacques, H. (1990), Monogr Atheroscler 16: 59-70. |
| Effects of dietary garlic on cholesterol metabolism in laying hens Chowdhury, S. R., S. D. Chowdhury, et al. (2002), Poult Sci 81(12): 1856-62. Abstract: Experiments were conducted to evaluate the potential for dietary garlic to influence egg yolk cholesterol concentrations and overall performance in different layer strains. Thirty-six, 28-wk-old, Hisex Brown,Isa Brown, Lohmann, Starcross, Babcock, and Starcross-579 strains (six hens per strain) were fed diets supplemented with 0 (control), 2, 4, 6, 8, or 10% sun-dried garlic paste for 6 wk. There were no differences (P > 0.05) among diets or strains in egg weight, egg mass, feed consumption, feed efficiency, and BW gain as averaged over 6 wk. Yolk weight, however, responded quadratically with increasing levels of dietary garlic and differed among strains (P < 0.05). Egg production and yolk weights were significantly higher in the Babcock strain in comparison with other strains. Serum and egg yolk cholesterol concentrations decreased linearly (P < 0.05) with increasing levels of dietary garlic. Serum and egg yolk cholesterol concentrations also differed among different strains (P < 0.05). It was concluded that garlic paste in the diets of laying hens reduced serum and yolk cholesterol concentrations. It was also concluded that dietary garlic paste had no adverse effects on layer performance. |
| Effects of dietary konjac mannan on serum and liver cholesterol levels and biliary bile acid composition in hamsters Shimizu, H., M. Yamauchi, et al. (1991), J Pharmacobiodyn 14(7): 371-5. Abstract: We studied the effect of dietary konjac mannan on serum and liver cholesterol levels and biliary bile acid composition in hamsters. In hamsters fed a control diet supplemented with konjac mannan for 6 weeks, serum and liver cholesterol levels were decreased significantly compared to hamsters fed the control diet. Addition of konjac mannan to the cholesterol-enriched diet reduced the elevation of serum cholesterol caused by feeding cholesterol. However, the absorption of cholesterol from the intestine was not changed between the four groups. The increase in the proportion of chenodeoxycholic acid in biliary bile acids was significantly enhanced in the hamsters fed both cholesterol and konjac mannan compared to hamsters fed the control diet. The change of the bile acid composition may suggest that konjac mannan inhibits the intestinal absorption of bile acids. |